Determining the Influence of pH on the Activity of Catalase Enzyme in Potato Tuber Cells

INTRODUCTIONEnzymes atomic number 18 vit every(prenominal)y important for every(prenominal) living organism, because without them the reactions within the cells would occur to slow to sustain life. but there ar many factors which affect the footstep of enzymes reactions. unmatchable of them is pH, which put to work on the enzyme activity testament be the subject of this investigation. Catalase of spud genus Tuber cells will be the enzyme employ in the experiment. RESEARCH QUESTIONWhat is the influence of diametrical set of pH on the activity of catalase in potato thermionic genus Tuber cells?VARIABLESIndependent: potato discs treatment (placing them into buffer solutions of different pH economic values, motley with henry hydrogen peroxide)Dependent: the activity of the catalase, reflected by the rate at which type O is evolved, measured at every snip the pH is changed. Controlled: whether the potato discs were chemically threaten, whether the catalase and hydrogen peroxide engrossment was the same in every sample ( whether the potato discs were accurately cut and whether they all came from the same potato, whether the same fresh hydrogen peroxide was use in all samples) and whether the temperature remained the same during the unit experiment. HYPOTHESISDifferent pH values influence the activity of catalase. It was obtained from the literature, that the range of pH values, at which catalase plant is just about 4 to 9, with an optimum pH of 7.

Bearing this in mind the following hypothesis has been make:If the potato discs are placed into a test tube with hydrogen peroxide and a buffer solution at pH approximately 7, then the activity of catal! ase will be the greatest. If the pH value is less or more than 7 the activity of the enzyme will be smaller. THEORYAn enzyme is a biological catalyst. This mean that its a substance, produced in living organisms, which speeds up a current reaction, because it lowers... If you want to labour a full essay, order it on our website: BestEssayCheap.com

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